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学术报告 2021年06月18日(星期五)上午10∶00 报告人:Prof. Xiuren Zhang
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学术报告 2021年06月18日(星期五)上午10∶00 报告人:Prof. Xiuren Zhang

题 目: History, current progress, and future direction of miRNA research
报告人: Prof. Xiuren Zhang,
             
Department of Biochemistry & Biophysics
              Institute for Plant Genomics & Biotechnology
              Texas A&M University
时 间: 2021年06月18日(星期五) 上午10∶00-11∶30
地 点: 中国农业大学  生命科学研究中心  一层报告厅(线上直播)

腾讯会议直播:https://meeting.tencent.com/l/uLV5XR0iPnUE

miRNAs are a group of small noncoding RNAs that are widely present in eukaryotes. miRNAs are loaded into AGO proteins to form RNA-induced silencing complexes (RISCs) to repress gene expression through target cleavage and/or translational repression. In metazoan miRNAs target more than 60% transcripts. Similarly in plants, they regulate essentially every aspect of growth and development as well as biotic and abiotic stress responses. miRNAs originate from primary transcripts (pri-miRNAs), which feature stem-loop structures. The pri-miRNAs are sequentially cleaved by Microprocessor that minimally consists of Dicer-like 1 (DCL1), a double-stranded (ds)RNA-binding protein, Hyponastic leaves 1(HYL1), and Serrate (SE) to eventually produce miRNA. Therefore, precise control of miRNA production and fine-tuning of homeostasis of miRNA accumulation warrant the functional accuracy and targeting efficacy of miRNAs. Similarly, precise controls of miRNA loading into different AGOs and miRNA decay are also critical for their regulatory functions in vivo. In the past years, many new components and genetic regulatory layers have been revealed to be involved in miRNA pathway. I will consolidate the discovery, and current progress in the miRNA field. I will also discuss on some newly emerging questions in the field. 



I. Personal Information:
Name:  Xiuren Zhang
Address:  Texas A&M University
Department of Biochemistry & Biophysics
Institute for Plant Genomics & Biotechnology
2128 TAMU
College Station, TX 77843-2128
Tel: 979.458.0596, fax: 979.845.9274
E-mail: xiuren.zhang@tamu.edu

II. Education:
Cornell University               Ph.D.  2003   Plant Biology
Auburn University, AL            M.S.   1999   Horticulture/Biotech
China Agricultural University    M.S.   1994   Horticulture
Wannan Agricultural College      BS.    1989   Economic Plant
 (now incorporated into University of Science and Technology of China)

III. Experience:

Positions and Employment
2018.9-    Professor, Department of Biochemistry & Biophysics and Institute for Plant Genomics & Biotechnology, Texas A&M University
2014.9-2018.8   Associate Professor, Department of Biochemistry & Biophysics and Institute for Plant Genomics & Biotechnology, Texas A&M University
2008.9-2014.8   Assistant Professor, Department of Biochemistry & Biophysics and Institute for Plant Genomics & Biotechnology, Texas A&M University
2003.9-2008.7   Labs of Plant Molecular Biology and RNA Biology, Rockefeller University,  NY, Postdoctoral Fellow
1994.7-1997.3   Assistant to Director, Beijing Academy of Agriculture & Forestry, China

Honors
2020   Presidential Impact Fellow
2019   Chancellor EDGES Fellow
2019   Agrilife Faculty Fellow
2016   Norman Borlaug Outstanding Faculty
2013   NSF CAREER award
1996   Distinguished young fellow in Municipality of Beijing, China
1991   Excellent administrator for Taihu County, Anhui, China

IV.  Publication lists: (* equal contribution; ** corresponding author; #, graduate students; $, undergraduate students)

1. Sun, D., and Zhang, X. HASTY moves to chromatin for miRNA production. Mol Plant. 2021 Jan 22:S1674-2052(21)00012-5. doi: 10.1016/j.molp.2021.01.012. PMID: 33493678.
2. Zhu, J., Li, C., Peng, X., and Zhang, X. Architecture of RNA influences plant biology. J Exp Bot. 2021 Jan 23:erab030. doi: 10.1093/jxb/erab030. PMID: 33484251
3. Sun, D., Ma, Z., Zhu, J., and Zhang, X. Identification and Quantification of Small RNAs. Methods Mol Biol. 2021;2200:225-254. doi: 10.1007/978-1-0716-0880-7_11. PMID: 33175381
4. Li. Y.*, Sun, D.*, Ma, Z., Yamaguchi, K.$, Wang, L, Zhong, S.  Yan, X., Shang, B., Nagashima, Y., Koiwa, H., Han, J., Xie, Q., Zhou, M., Wang, Z.**, and Zhang, X.**, 2020. Degradation of Serrate via ubiquitin-independent 20S proteasome to survey RNA metabolism. Nature Plants. 2020 Aug;6(8):970-982. doi: 10.1038/s41477-020-0721-4
5. Kumar, V., Ivens, A., Goodall, Z., Meehan, J., Doharey, P.K., Hillhouse, A., Hurtado, D.O., Cai, J.J., Zhang, X., Schnaufer, A., and Cruz-Reyes, J. 2020. Site-specific and mRNA-specific control of accurate mRNA editing by a helicase complex in trypanosomes. RNA. doi: 10.1261/rna.076513.120. Online ahead of print. PMID: 32873716
6. Sun, D#., Ma, Z., Zhu, J., and Zhang, X.**. 2020. Identification and Quantification of Small Non-coding RNAs. 2019. Arabidopsis Book (In press).
7. Nagashima, Y., Ma, Z., Zhang, X., von Schaewen, A., and Koiwa, H., 2020.  Lack of endoplasmic reticulum quality control (ERQC) promotes tonoplast (TP) targeting of KORRIGAN 1 (KOR1). Plant Signal Behav. 2020 Mar 20:1744348. doi: 10.1080/15592324.2020.1744348.
8. Nagashima, Y., Ma, Z., Liu, X., Qian, X., Zhang, X., von Schaewen, A., and Koiwa H. 2020. Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1. Plant Cell. 2020 Feb;32(2):470-485. doi: 10.1105/tpc.19.00714. Epub 2019 Dec 18.
9. Hu, T., Huang, C., He, Y., Castillo-González, C.#, Gui, X., Wang, Y., Zhang, X.**, Zhou, X.**. 2019. βC1 protein encoded in geminivirus satellite concertedly targets MKK2 and MPK4 to counter host defense. PLoS Pathog. Apr 18;15(4):e1007728. doi: 10.1371/journal.ppat.1007728.
10. Wang, Z*., Wang, Y.*, Wang, T., Zhang, Y.**, and Zhang, X.**. 2019.  Genome-wide probing RNA structure with the modified DMS-MaPseq in Arabidopsis. Methods 155:30-40. doi: 10.1016/j.ymeth.2018.11.018.
11. Castillo-Gonzalez, C. #, and Zhang, X.**. 2018. The Trojan Horse of the Plant Kingdom. Cell Host & Microbe. 24(1):1-3. doi: 10.1016/j.chom.2018.06.015.
12. Ma, Z., Castillo-González, C. #, Wang, Z., Sun, D. #, Hu, X., Shen, X., Potok, M.E., and Zhang, X.** Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression. Developmental Cell. 45(6):769-784.e6. doi: 10.1016/j.devcel.2018.05.023.
13. Ma, Z., and Zhang, X.**. 2018. Actions of plant Argonautes: predictable or unpredictable? Curr Opin Plant Biol. 45:59-67. doi: 10.1016/j.pbi.2018.05.007.
14. Wang, Z., Ma, Z.*, Castillo-González, C. #*, Sun, D. #, Li, Y. #, Yu, B., Zhao, B., Li, P., and Zhang, X.**. 2018.  SWI/SNF subunit CHR2 remodels pri-miRNAs via SE to inhibit miRNA production. Nature. 557(7706):516-521.
15. Castillo-Gonzalez, C. #, and Zhang, X. 2018. Transactivator: the new face of Arabidopsis AGO1. Developmental Cell. 44(3): 277-279
16. Njaci, I. #, Williams, B., Castillo-González, C.#, Dickman, M., Zhang, X., and Mundree, S. 2018. Genome-wide investigation of the role of microRNAs in desiccation tolerance in the resurrection grass Tripogon loliiformis. Plants. 7(3), 68. Doing: 10.3390/plants7030068
17. Mei, Y., Yang, X., Huang, C#., Zhang, X., and Zhou, X. 2018. Tomato leaf curl Yunnan virus-encoded C4 induces cell division through enhancing stability of Cyclin D 1.1 via impairing NbSKη -mediated phosphorylation in Nicotiana benthamiana. PLoS Pathog. 14(1): e1006789.
18. Zhang, X.**. 2017. Tough GC beats transgene silencing. Nature Plants. 2017 Nov; 3(11): 850-851. doi: 10.1038/s41477-017-0048-y.
19. Fukudome, A., Sun, D#. Zhang, X., and Koiwa, H.  2017. Salt-stress and CTD phosphatase-like 4 mediate transcriptional switching of snRNA to mRNA in Arabidopsis thaliana. Plant Cell. 2017 Nov 1. pii: tpc.00331.2017. doi: 10.1105/tpc.17.00331.
20. Zhang, Z.*, Hu F.* #, Sung, M. W#., Shu, C, Castillo, C. #, Koiwa, H., Dickman, M., Li, P.** and Zhang, X.**. 2017. RISC-Interacting Clearing 3'- 5' Exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis. eLife. doi: 10.7554/eLife.24466 (the paper was favorably reviewed as “tour de force” work).
21. Zhang, Z.*, Guo, X.*, Ge, C#*., Ma, Z., Jiang, M. #, Li, T., Koiwa H., Yang, S.K., and Zhang, X.**. 2017. KETCH1 (Karyopherin Enabling the Transport of the Cytoplasmic HYL1) imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis. PNAS. 114(15):4011-4016
22. Li, S*., Castillo-Gonzalez, C*#., Yu, B**., and Zhang, X.**. 2017. The functions of plant small RNAs in development and in stress responses. Plant J. 90(4):654-670
23. Guo, X., Ma, Z., Zhang, Z. Cheng, L. Zhang, X.**, and Li, T**., 2017. Small RNA-Sequencing Links Physiological Changes and RdDM Process to Vegetative-to-Floral Transition in Apple. Front. Plant Sci. 10.3389/fpls.2017.00873
24. Zhang, Z.*, Liu, X.*, Guo, X., Wang, X-J.** and Zhang, X.**. 2016. Arabidopsis AGO3 predominantly recruits 24-nt small RNAs to regulate epigenetic silencing. Nature Plants. 16049 (2016) doi:10.1038/nplants.2016.49 (Featured paper).
25. Zhou, Y., Honda, M., Zhu, H., Zhang, Z., Guo, X., Li, T., Li, Z., Peng, X., Nakajima, K., Duan, L., and Zhang X.** 2015. Spatiotemporal Sequestration of miR165/166 by Arabidopsis Argonaute10 Promotes Shoot Apical Meristem Maintenance. Cell Report. S2211-1247.
26. Wang, T., Castillo-González, C. #, You, L., Li, R., Wen, L., Zhu, H. **, and Zhang, X.**. 2015. In vitro reconstitution assay of miRNA biogenesis by Arabidopsis DCL1. Bio-protocol 5(8): e1454.
27. Castillo-González, C. #, Liu, X., Huang, C., Zhao, C., Hu, T., Sun, F$., Ma, Z., Zhou, Y., Zhou, X., Wang, X., and Zhang, X.**. 2015. Geminivirus-encoded TrAP suppressor inhibits the histone methyltransferase SUVH4/KYP to counter host defense. eLife. 2015 Sep 7;4. doi: 10.7554/eLife.06671. (Featured in eLife and selected by Nature for research highlight)
28. Williams, B., Njaci, I. #, Moghaddam, L., Long, H., Dickman, M., Zhang, X., and Mundree, S.**. 2015. Trehalose Accumulation Triggers Autophagy during Plant Desiccation. PLoS Genet. 2015 Dec 3;11(12):e1005705
29. Zhu, H., Zhou, Y., Castillo, C. #, Lu, A$., Zhao, Y., Duan, L., Li, Z., Wang, XJ and Zhang, X.**.  2013. Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1. Nature Struc. Mol. Biol. 20:1106-1115 (Cover Story).
30. Zhang, Z. and Zhang, X.**. 2012. Argonautes fight for miRNAs to regulate stem cell activity in plants. Current Opinion in Plant Biology. 15:652-658 (Cover).
31. Zhu, H.*, Hu, F.* #, Wang, R.*, Zhou, X., Sze, S., Liou, L. $, Barefoot, A. $, Dickman, M.,and Zhang, X.**.  2011. The Arabidopsis Argonaute 10 specifically recruits miR166/165 to maintain shoot apical meristem. Cell. 145:242-256.  (Featured article; Top 5 Cell paperflick in 2011)
32. Wang, H.*, Zhang, X.*, Liu, J., Kiba, T., Ojo, T. $, Hafner, M., Tuschl, T., Nam-Hai Chua** and Wang, X.**. 2011. Deep sequencing of small RNAs specifically associated with AGO1 and AGO4 uncovers new AGO functions. The Plant J. 67, 292–304.  PMCID: PMC3135789
 

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