课题组长

English

杨淑华     杨淑华 实验室

职称:教授
联系电话:86-10-62734838 (O); 86-10-62734390 (L)
E-mail: yangshuhua@cau.edu.cn
研究方向:植物响应低温胁迫的分子机制

教育背景
1987年9月-1991年7月,南开大学,理学学士学位
1991年9月-1994年7月,南开大学,理学硕士学位
1998年7月-2002年1月,新加坡国立大学,博士学位


工作经历
1994年7月-1998年6月,南开大学,助理研究员
2002年2月-2004年12月,美国康奈尔大学,博士后
2005年1月-至今,中国农业大学,教授

2005年入选教育部“新世纪优秀人才支持计划”
2012年获得“国家杰出青年科学基金”资助
2014年获教育部“长江学者”特聘教授

主要研究方向
研究方向:植物响应低温胁迫的分子机制
       作为外界主要环境因子,温度影响植物的生长发育、植被的分布以及农作物的产量。我们实验室主要是通过分子生物学、遗传学和生物化学等手段,研究植物应答低温胁迫的信号转导途径、低温信号与植物激素信号之间的交互作用,以及低温信号与生物胁迫信号之间的交互作用等。
 
1. 植物响应零下低温胁迫的分子调控研究
        在植物抗冻性研究方面,我们建立了稳定的冻相关突变体的筛选体系。通过该体系,我们发现蛋白激酶CRPK1(Cold-responsive protein kinase 1)参与负向调控低温信号途径关键转录因子CBF依赖的信号转导。低温激活定位于细胞膜上的CRPK1,后者磷酸化细胞质中的14-3-3蛋白,诱导其进入细胞核中,与CBF1/3蛋白互作,介导CBF1/3蛋白的泛素化降解,从而负调控植物对低温的应答。该研究不仅揭示了低温信号从细胞膜传递到细胞核中的负调控机制,也阐明了CBF蛋白翻译后修饰的调控机制(Liu et al., 2017, Mol Cell)。而蛋白激酶OST1在植物低温应答中发挥重要的正向调控作用。OST1激酶活性被低温迅速诱导,激活状态的OST1将ICE1磷酸化,抑制ICE1的泛素化降解,从而正调控植物的抗冻性(Ding et al., 2015, Dev Cell)。
    在低温信号和植物激素信号的交叉互作方面,我们发现乙烯信号通路关键组分均参与低温应答。转录因子EIN3蛋白直接负调控低温调节基因CBF和细胞分裂素信号负调控因子A型ARR基因的表达,从而负调控拟南芥的抗冻性。该研究明确了乙烯在植物抗低温中的作用,同时也揭示乙烯和细胞分裂素信号之间交互作用的分子机制(Shi et al., 2012, Plant Cell)。另一个重要植物激素油菜素内酯信号通路关键的蛋白激酶BIN2和转录因子BZR1/BES1通过依赖和不依赖于CBF的方式调控下游一系列基因的表达,从而正向调节植物的抗冻能力(Li et al., 2017, Mol Plant)。利用CRISPR技术,构建了cbfs三缺失突变体,明确了CBFs在低温驯化中的关键作用及CBFs的靶基因,并为低温信号研究提供了理想的遗传材料 (Jia et al., 2016, New Phytol)。

2. 植物响应零上低温胁迫的分子调控研究
       我们的研究发现拟南芥防卫反应中起重要作用的抗病相关基因CHS1RPP4/CHS2CHS3和LSD1/CHS4参与不同温度下植物生长的稳态平衡调控 (Wang et al., 2013, Plant J; Wang et al., 2011, Plant J; Huang et al., 2010, Plant Physiol; Yang et al., 2010, Plant J; Huang et al., 2010, New Phytol)。研究揭示TIR-NB类蛋白CHS1通过与R蛋白SOC3互作,蛋白磷酸酶IBR5和伴侣蛋白HSP90分别与R蛋白CHS3和CHS2互作,调控R蛋白的活性,从而调控R介导的温度依赖的细胞死亡和防卫反应 (Zhang et al., 2016, New Phytol; Liu et al., 2015, PLoS Genet; Bao et al., 2014, New Phytol)。
       研究发现拟南芥磷脂结合蛋白BON1在调控温度依赖的生长发育中是一个关键调控因子,BON1与两个类受体蛋白激酶BIR1和BAK1互作,可能通过BAK1对BIR1和BON1的磷酸化,作用于同一条信号通路,协同调控温度依赖的植物生长发育和防卫反应 (Wang et al., 2011, Plant J; Yang et al., 2007, Plant Physiol; Yang et al., 2006, Plant J; Yang and Hua, 2004, Plant Cell)。
发表论文/论著    (*通讯作者)

     Jiang B#, Shi Y#, Zhang X, Xin X, Qi L, Guo H, Li J*, Yang S* (2017) PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis. Proc Natl Acad Sci U S A. 114: E6695-E6702.

     Liu Z, Jia Y, Ding Y, Shi Y, Zhang X, Li Z, Guo Y, Gong Z, Yang S* (2017) Plasma membrane CRPK1-mediated phosphorylation of 14-3-3 proteins induces their nuclear import to fine-tune CBF signaling during cold response. Mol Cell 66: 117-128.  (Previewed in the same issue of Mol Cell)

     Li H#, Ye K#, Shi Y, Zhang X, Yang S* (2017) BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis. Mol Plant 10: 545-559. (#These authors contributed equally) (Spotlighted in the same issue of Mol Plant)

     Zhang Z#, Li J#, Pan Y#, Li J#, Zhou L#, Shi H#, Zeng Y#, Guo H, Yang S, Zheng W, Yu J, Sun X, Li G, Ding Y, Ma L, Shen S, Dai Ln, Zhang H, Yang S, Guo Y, Li Z* (2017) Natural variation in CTB4a enhances rice adaptation to cold habitats. Nat Commun 8:14788. Doi: 10.1038/ncomms14788.

     Zhang Y#, Wang Y#, Liu J, Ding Y, Wang S, Zhang X, Liu Y, Yang S* (2017) Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3. New Phytol 213:1330-1345.

     Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S* (2016) The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol 212: 345-353. (Commentary in the same issue of New Phytol)

     Tong M, Kotur T, Liang W, Vogelmann K, Kleine T, Leister D, Brieske C, Yang S, Lüdke D, Wiermer M, Zhang Y, Li X*, Hoth S*. (2017) E3 ligase SAUL1 serves as a positive regulator of PAMP-triggered immunity and its homeostasis is monitored by immune receptor SOC3. New Phytol 215:1516-1532.

     Huang X, Zhang X, Gong Z, Yang S, Shi Y* (2016) ABI4 represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis. Plant J 89: 354-365.

     Yu F, Lou L, Tian M, Li Q, Ding Y, Wu Y, Belda-Palazon B, Rodrigue P L, Yang S, Xie Q* (2016) ESCRT-I component VPS23A affects ABA signaling by recognizing ABA receptors for endosomal degradation. Mol Plant 9: 1570-1582.

     Ding Y, Li H, Zhang X, Xie Q, Gong Z, Yang S* (2015) OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis. Dev Cell 32: 278-289. (Previewed in the same issue of Mol Cell)

     Liu J#, Yang H#, Bao F, Ao K, Zhang X, Zhang Y, Yang S* (2015) IBR5 modulates temperature-dependent, R protein CHS3-mediated defense responses in Arabidopsis. PLoS Genet 11, e1005584. (#These authors contributed equally)

     Kong L, Cheng J, Zhu Y, Ding Y, Meng J, Chen Z, Xie Q, Guo Y, Li J, Yang S, Gong Z* (2015) Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases. Nat Commun 6: 8630. Doi: 10.1038/ncomms9630.

    Ji H, Wang Y, Cloix C, Li K, Jenkin G, Wang S, Shang Z, Shi Y, Yang S, Li X* (2015) The Arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis. PLoS Genet 11, e1005471.

     Shi Y#, Ding Y#, Yang S* (2015) Cold signal transduction and its interplay with phytohormones during cold acclimation. Plant Cell Physiol 56: 7-15. (#These authors contributed equally)

     Bao F, Huang X, Zhu C, Zhang X, Li X, Yang S* (2014) Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol 202: 1320-1334.

     Song S, Huang H, Gao H, Wang J, Wu D, Liu X, Yang S, Zhai Q, Li C, Qi T, Xie D* (2014) Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell 26: 263-279.

     Wang Y, Zhang Y, Wang Z, Zhang X, Yang S* (2013) A missense mutation in CHS1, a TIR-NB protein, induces chilling sensitivity in Arabidopsis. Plant J 75: 553-565.

     Shi Y, Wang Z, Meng P, Tian S, Zhang X, Yang S* (2013) The glutamate carboxypeptidase AMP1 mediates ABA and abiotic stress responses in Arabidopsis. New Phytol 199: 135-150.

     Guo L, Yang H, Zhang X, Yang S* (2013) Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis. J Exp Bot 64: 1755-1767.

     Li J#, Mu J#, Bai J#, Fu F, Zou T, An F, Zhang J, Jing H, Wang Q, Yang S, Zuo J* (2013) PARAQUAT RESISTANT 1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat. Plant Physiol 162: 470-483. (#These authors contributed equally)

     Shi Y#, Tian S#, Hou L, Huang X, Zhang X, Guo H, Yang S* (2012) Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. Plant Cell 24: 2578-2595. (#These authors contributed equally)

     Wang Z#, Meng P#, Zhang X, Ren D, Yang S* (2011) BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis. Plant J 67: 1081-1093. (#These authors contributed equally)

     Huang X, Li J, Bao F, Zhang X, Yang S* (2010) A gain-of-function mutation in the Arabidopsis disease resistance gene RPP4 confers sensitivity to low temperature. Plant Physiol 154: 796-809.

     Yang H, Shi Y, Liu J, Guo L, Zhang X, Yang S* (2010) A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis. Plant J 63: 283-296.

     Huang X, Li Y, Zhang X, Zuo J, Yang S* (2010) The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death. New Phytol 187: 301-312 (Cover story).

     Liu D, Gong Q, Ma Y, Li P, Li J, Yang S, Yuan L, Yu Y, Pan D, Xu F, Wang NN* (2010) cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis. J Exp Bot 61: 1655-1669.

     Huang X, Zhang X, Yang S* (2009) A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis. Cell Res 19: 1205-1216.

     Gou M, Su N, Zheng J, Huai J, Wu G, Zhao J, He J, Tang D, Yang S, Wang G* (2009) An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis. Plant J 60: 757-770.

     Li Y#, Yang S#, Yang H, Hua J* (2007) The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors. Mol Plant Microbe Interact 20: 1449-1456. (#These authors contributed equally)

     Yang H, Yang S, Li Y, Hua J* (2007) The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death. Plant Physiol 145: 135-146.

     Chai MF, Wei PC, Chen QJ, An R, Chen YM, Chen J, Yang S*, Wang XC* (2006) NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis. Plant J 47: 665-674.

      Yang S, Yang H, Grisafi P, Sanchatjate S, Fink GR, Sun Q, Hua J* (2006) The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis. Plant J 45: 166-179.

     Yang S, and Hua J* (2004) A haplotype-specific resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis. Plant Cell 16: 1060-1071.

     Yang S*, Yu H, Xu Y, Goh CJ* (2003) Investigation of cytokinin-deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1. FEBS Lett 555: 291-296.

     Yang S, Yu H, Goh CJ* (2003) Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid. Plant Mol Biol 51: 237-248.

     Yang S, Yu H, Goh CJ* (2002) Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter. J Exp Bot 53: 1899-1907.

     Yu H, Yang S, Goh CJ* (2002) Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory elements. Plant Mol Biol 49: 225-237.

     Yu H, Yang S, Goh CJ* (2001) Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1. Plant Cell Rep 20: 301-305.

     Yu H, Yang S, Goh CJ* (2000) DOH1, a class 1 knox Gene, is required for maintenance of the basic plant architecture and floral transition in orchid. Plant Cell 12: 2143-2160. 

     丁杨林,施怡婷,杨淑华* (2015)  植物响应低温胁迫的分子机制研究. 生命科学. 27: 398-405.
     邓 岩,王兴春,杨淑华*,左建儒* (2006) 细胞分裂素:代谢、信号转导、交叉反应与农艺性状改良. 植物学通报. 23: 478-498.

专著

      Shi Y, Yang S* (2014) ABA regulation of the cold stress response in plants. In Zhang D (ed) Abscisic Acid: Metabolism, Transport and Signaling. Springer, pp337-363.
      Yu H, Dhavale T, Yang S (2006) Molecular mechanisms of hormone functions in flowering In Teixeira da Silva JA (ed) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Ed 1 Vol 1 Global Science Books, London, UK, pp 25-32.
      任勃,王兴春,冯健,杨淑华,左建儒 (2012)  细胞分裂素. 植物激素作用的分子机理 (许智宏,薛红卫主编) 上海科学技术出版社.
      武维华,张蜀秋,袁明,张军,韩玉珍,李颖章,叶德,杨淑华,王学臣 (2008) 植物生理学 (第二版) 科学出版社.