细胞骨架在植物细胞顶端生长中的作用
   细胞如何保持其内畅通运输是Science杂志提出的最具挑战性的125个重要科学问题之一。研究表明，微丝骨架与肌球蛋白myosin XI在植物细胞原生质流动与细胞器运动方面起主要作用；但目前植物微丝骨架及其马达蛋白在细胞器运动方面的具体机制尚有待深入研究。我们通过对花粉管内单体肌动蛋白标记发现，在缺乏微丝束的花粉管顶端10-20微米区域，存在明显的单体肌动蛋白浓度梯度。Caffeine处理后，花粉管顶端单体肌动蛋白浓度梯度消失，微丝束前移到花粉管顶端。因此，我们提出：花粉管顶端微丝束的缺乏是由于其被解聚的结果，而花粉管顶端的高浓度的钙离子很可能是导致微丝束解聚的重要因素；此外，我们还发现环绕的微丝束是花粉管内微丝的主要存在方式，并推测其可为双向的原生质流动提供轨道（Li et al., 2001）。另外，我们发现单体肌动蛋白浓度梯度的形成与维持在根毛细胞的顶端生长中起重要作用（He et al., 2006）,根毛内也存在环绕微丝束（Zhang et al., 2014）。最近，我们课题组研究发现，拟南芥肌球蛋白Myo11C2与花粉管线粒体、过氧化物酶体和高尔基体存在部分共定位，并在线粒体运动、分布及分泌囊泡顶端运输积累等方面起重要作用(Wang et al., 2020)。目前，本实验室正利用分子细胞生物学等技术，从多方面对微丝骨架及其马达蛋白在植物细胞器运动及顶端生长方面的分子机制进行深入研究。

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