课题组长

English
毛同林         毛同林 实验室
职称:教授
联系电话:86-10-62732330 (O);86-10-62734396 (L)
E-mail:
maotonglin@cau.edu.cn
研究方向:细胞骨架与植物生长和细胞形态建成的调控机制

毛同林,博士,教授,博士生导师
    1996年毕业于内蒙古农牧学院农学系,获学士学位;1999年获中国农业大学植物生理学与生物化学专业,理学硕士学位;1999年-2005年在中国农业科学院工作;2005年获中国农业大学植物学专业,理学博士学位,同年留校工作。2008年至2009年美国斯坦福大学卡耐基研究所,访问学者。
    2012 年获得 “国家优秀青年科学基金”资助  (2013-2015);2012年入选教育部“新世纪优秀人才支持计划”;2016年获得“国家杰出青年科学基金”资助(2017-2021)。近年来以通讯作者在 PNAS, Plant Cell,Plant Physiol,J Cell Sci等刊物发表多篇研究论文。

主要研究方向

植物细胞微管骨架(Microtubules, MTs)在调控植物生长发育、细胞形态建成以及响应逆境胁迫等生理过程中起着重要的作用。本课题组通过细胞生物学、分子生物学、遗传学和生物化学等手段,研究细胞微管骨架响应光、植物激素以及非生物逆境等信号分子调控植物细胞生长和生存的生物学机制。我们一方面关注生长发育和环境信号如何传递至微管骨架以及不同的信号途径如何调控微管结合蛋白;另一方面探索微管骨架调控上游信号的新机制和新途径。
1、植物细胞微管骨架调控下胚轴生长的分子机制。下胚轴细胞生长的调控一直是植物生理学中的研究热点问题。大量的研究表明光、植物激素(如生长素,赤霉素,油菜素内酯)以及钙离子等参与了下胚轴细胞生长的调控。本课题组重点研究了微管骨架及其调节蛋白在调控下胚轴细胞伸长生长中的生理学功能,以及它们与光、植物激素和钙离子等上游调控因子之间的功能关系,包括钙离子对微管去稳定蛋白MDP25功能的调控,植物激素油菜素内酯对微管去稳定蛋白MDP40转录水平的调控以及26S蛋白酶体途径对微管稳定因子WDL3蛋白水平的调控等诸多调控途径。
2. 细胞骨架参与非生物逆境的功能分析。植物在适应非生物逆境过程中,需要调控自身的生长、发育以使其完成生命周期,而细胞骨架在其中起着重要的作用。我们实验室致力于鉴定参与该过程的重要的骨架调控蛋白,并试图探讨它们调控的分子机制。

发表论文/论著    (*通讯作者)

(*Corresponding author; #These authors contributed equally)

1.
Xuan Zhao#, Liru Dou#, Zhizhong Gong, Xiangfeng Wang*, and Tonglin Mao (2018) BES1 hinders ABSCISIC ACID INSENSITIVE5 and promotes seed germination in Arabidopsis. New Phytol Doi.org/10.1111/nph.15437
2. Liru Dou, KaiKai He, Takumi Higaki, Xiangfeng Wang, and Tonglin Mao* (2018) Ethylene signaling modulates cortical microtubule reassembly in response to salt stress. Plant Physiol 176: 2071–2081
3. Qianqian Ma#, Xiaohong Wang#, Jingbo Sun#, and Tonglin Mao* (2018) Coordinated regulation of hypocotyl cell elongation by light and ethylene through a microtubule destabilizing protein. Plant Physiol 176: 678–690
4. Na Lian#, Xiaomin Liu#, Xiaohong Wang#, Yangyang Zhou, Hong Li, Jigang Li* and Tonglin Mao* (2017) COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation. Proc Natl Acad Sci USA 114: 12321–12326
5. Qun Zhang*#, Ping Song, Yana Qu, Peipei Wang, Qianru Jia, Liang Guo, Chuanpeng Zhang, Tonglin Mao, Ming Yuan, Xuemin Wang, and Wenhua Zhang* (2017) Phospholipase Dδ negatively regulates plant thermotolerance by destabilizing cortical microtubules in Arabidopsis. Plant Cell Environ 40: 2220–2235
6. Jingen Zhu#,Qiong Nan#, Tao Qin#, Dong Qian, Tonglin Mao, Shunjie Yuan, Xiaorong Wu, Yue Niu, Qifeng Bai, Lizhe An, and Yun Xiang* (2017) Higher-ordered actin structures remodeled by Arabidopsis ACTIN-DEPOLYMERIZING FACTOR5 are important for pollen germination and pollen tube growth.Mol Plant 10: 1065–1081
7. Qianqian Ma#,Jingbo Sun#,and Tonglin Mao* (2016) Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls. J Cell Sci 129: 2043–2051
8. Liangliang Chen#, Yuancheng Peng#, Juan Tian, Xiaohong Wang, Zhaosheng Kong, Tonglin Mao, Ming Yuan, and Yunhai Li* (2016) TCS1, a Microtubule-Binding Protein, interacts with KCBP/ZWICHEL to regulate trichome cell shape in Arabidopsis thaliana. PLoS Genet 12: e1006266
9. Jingbo Sun#, Qianqian Ma#, and Tonglin Mao* (2015) Ethylene regulates Arabidopsis Microtubule-Associated Protein WDL5 in etiolated hypocotyl elongation. Plant Physiol 169: 325–337 
10. Yuan Zhou#, Shiping Yang, Tonglin Mao, and Ziding Zhang* (2015) MAPanalyzer: a novel online tool for analyzing microtubule-associated proteins. Database (Oxford) pii: bav108
11. Xiaoyu Wang#, Xianling Wang#, Qingnan Hu, Xuemei Dai, Hainan Tian, Kaijie Zheng, Xiaoping Wang, Tonglin Mao, Jin‐Gui Chen, and Shucai Wang* (2015) Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis. Plant J 83: 300–311
12. Tao Qin#, Xiaomin Liu#, Jiejie Li, Jingbo Sun, Leina Song, and Tonglin Mao* (2014) Arabidopsis Microtubule-Destabilizing Protein 25 Functions in Pollen Tube Growth by Severing Actin Filaments. Plant Cell 26: 325–339
13. Xiaomin Liu#, Tao Qin#, Qianqian Ma, Jingbo Sun, Ziqiang Liu, Ming Yuan, and Tonglin Mao* (2013) Light-regulated hypocotyl elongation involves proteasome-dependent degradation of the microtubule regulatory protein WDL3 in Arabidopsis. Plant Cell 25: 1740–1755
14. Liang Wang#, Tian Piao#, Muqing Cao#, Tao Qin, Lei Huang, Haiteng Deng, Tonglin Mao, and Junmin Pan* (2013) Flagellar regeneration requires cytoplasmic microtubule depolymerization and kinesin-13. J Cell Sci 126: 1531–1540
15. Xianling Wang#, Jin Zhang#, MingYuan, David W. Ehrhardt, Zhiyong Wang, and Tonglin Mao* (2012) Arabidopsis MICROTUBULE DESTABILIZING PROTEIN40 is involved in brassinosteroid regulation of hypocotyl elongation. Plant Cell 24: 4012–4025
16. Tao Qin#, Jiejie Li, Ming Yuan,and Tonglin Mao* (2012) Characterization of the Role of Calcium in Regulating the Microtubule-Destabilizing Activity of MDP25. Plant Signal Behav 7: 708–710
17. Qun Zhang#, Feng Lin, Tonglin Mao, Jianing Nie, Min Yan, Ming Yuan,and Wenhua Zhang* (2012) Phosphatidic Acid Regulates Microtubule Organization by Interacting with MAP65-1 in Response to Salt Stress in ArabidopsisPlant Cell 24: 4555–4576
18. Jiejie Li#, Xianling Wang#, Tao Qin#, Yan Zhang#, Xiaomin Liu, Jingbo Sun, Yuan Zhou, Lei Zhu, Ziding Zhang, Ming Yuan, and Tonglin Mao* (2011) MDP25, A novel calcium regulatory protein, mediates hypocotyl cell elongation by destabilizing cortical microtubules in Arabidopsis. Plant Cell  23: 4411-4427 
19. Yang Zhao#, Shuangshuang Zhao#,Tonglin Mao, Xiaolu Qu, Wanhong Cao, Li Zhang, Wei  Zhang, Liu He, Sidi Li, Sulin Ren, Jinfeng Zhao, Guoli Zhu, Shanjin Huang, Keqiong Ye, Ming Yuan, and Yan Guo* (2011) The Plant-Specific Actin Binding Protein SCAB1 Stabilizes Actin Filaments and Regulates Stomatal Movement in Arabidopsis. Plant Cell 23:2314–2330
20. Qiutao Meng#, Jizhou Du#, Jiejie Li, Xiaomei Lü, Xian Zeng, Ming Yuan and Tonglin Mao* (2010) Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules. Plant Mol Biol 74:537–547
21. Hua Li#, Xian Zeng#, Zi-Qiang Liu, Qiu-Tao Meng, Ming Yuan and Tonglin Mao*(2009) Arabidopsis Microtubule-Associated Protein AtMAP65-2 acts as a microtubule stabilizer. Plant Mol Biol 69: 313–324
22. Feng Wang#, Yanping Jing#, Zhen Wang, Tonglin Mao, Jozef Šamaj, Ming Yuan, and Haiyun Ren* (2009) Arabidopsis profilin isoforms, PRF1 and PRF2 show distinctive binding activities and subcellular distributions. J Integr Plant Biol 51: 113–121
23. Hua Li#, Tonglin Mao#, Ziding Zhang, and Ming Yuan* (2007) The AtMAP65-1 cross-bridge between microtubules is formed by one dimer. Plant Cell Physiol 48: 866–874
24. Hua Li, Ming Yuan, Tonglin Mao* (2007) AtMAP65-1 Binds to Tubulin Dimers to Promote Tubulin Assembly. J Biochem Mol Biol 40: 218–225
25. Tonglin Mao#, Lifeng Jin, Hua Li, Bo Liu, and Ming Yuan* (2005) Two Microtubule-Associated Proteins of the Arabidopsis MAP65 Family Function Differently on Microtubules.Plant Physiol 138: 654–662