课题组长

English
李继刚         李继刚 实验室

职称:
教授
联系电话:86-10-62734830 (O);86-10-62734853 (L)
E-mail:jigangli@cau.edu.cn
研究方向:植物光形态建成与逆境应答

李继刚,博士,教授,博士生导师。植物生理学与生物化学国家重点实验室项目负责人(Principal Investigator)。2006年获得北京大学理学博士学位,2007年-2013年在美国耶鲁大学分子、细胞与发育生物学系(Department of Molecular, Cellular and Developmental Biology, Yale University)从事博士后研究。2013年以“拔尖人才”引进中国农业大学,任生物学院教授。2014年入选中组部第五批“青年千人计划”。

主要研究方向

研究方向:植物光形态建成与逆境应答
光不但为植物提供光合作用所需的能量,而且还作为重要的环境信号,调控植物生长和发育的多个过程,比如种子萌发、幼苗去黄化(seedling de-etiolation)、避荫反应、开花,等等。植物通过不同家族的光受体(photoreceptors)感受不同波长的光,其中光敏色素(phytochromes)特异感受红光(600-700 nm)与远红光(700-750 nm)。拟南芥的基因组一共编码5个光敏色素蛋白(phyA-phyE),其中phyA是主要的远红光受体,phyB是主要的红光受体。本实验室通过结合分子、生化和细胞生物学的方法,研究远红光信号如何在植物中进行传递,以及光信号与植物逆境应答反应的交互作用(cross-talk)等。
1.光敏色素A(phyA)信号传导途径的分子机理
野生型(wild-type, WT)拟南芥的幼苗在黑暗下发育出较长的下胚轴(hypocotyl)和闭合的子叶(cotyledon),但是在远红光下则展现出较短的下胚轴和张开的子叶;然而,phyA突变体无法感受到远红光信号,在远红光下发育出黑暗下的表型,即具有较长的下胚轴和闭合的子叶(见下图)。

图1.野生型和phyA突变体幼苗在黑暗和远红光下的表型

经过多年的研究,尽管人们已经发现了phyA信号传导途径的几个关键调控因子,但是已知信号通路中还缺少很多的连接点,暗示还有许多重要的调控因子未被发现。但是,传统的通过筛选T-DNA插入突变体的方法已经很难筛选到新的调控因子。因此,本实验室将尝试通过筛选activation-tagging的拟南芥突变体库,筛选在远红光下具有长下胚轴表型的突变体。确定突变基因后,将尝试阐述突变基因在phyA信号传导途径中的工作机理。
2.光信号传导与植物逆境应答反应的交互作用
已有的研究表明,光信号传导途径与植物逆境应答反应存在着交互作用;然而,人们对此知之甚少。本实验室将对光信号传导途径的多个突变体进行逆境(盐、旱和低温)处理,从而推断哪些突变基因可能参与了光与逆境应答反应的交互作用。随后,通过一系列分子、生化和细胞生物学的方法,我们将尝试阐述这些基因介导光与逆境交互作用的分子机理。

发表论文/论著    (*通讯作者)

 

1)      Jiang B, Shi Y, Zhang X, Xin X, Qi L, Guo H, Li J*, Yang S* (2017). PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis. Proc Natl Acad Sci USA doi: 10.1073/pnas.1706226114.

2)      Li J, Wu Y, Xie Q, Gong Z (2017) Abscisic acid. In: Li J, Li C, Smith SM (Eds.), Hormone Metabolism and Signaling in Plants. Academic Press, United States of America, 161-202.

3)      Xu D, Jiang Y, Li J, Lin F, Holm M, Deng XW (2016). BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation. Proc Natl Acad Sci USA 113:7655-7660.

4)      Wang Z, Li N, Li J, Dunlap JC, Trail F, Townsend JP (2016). The fast-evolving phy-2 gene modulates sexual development in response to light in the model fungus Neurospora crassa. MBio 7:e02148-15.

5)      Kong L, Cheng J, Zhu Y, Ding Y, Meng J, Chen Z, Xie Q, Guo Y, Li J, Yang S, Gong Z (2015). Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases. Nat Commun 6:8630.

6)      Yang L, Li B, Zheng XY, Li J, Yang M, Dong X, He G, An C, Deng XW (2015). Salicylic acid biosynthesis is enhanced and contributes to increased biotrophic pathogen resistance in Arabidopsis hybrids. Nat Commun 6:7309.

7)      Wang C, Zheng Y, Zhao Y, Zhao Y, Li J, Guo Y (2015). SCAB3 is required for reorganization of actin filaments during light quality changes. J Genet Genomics 42:161-168.

8)      Zhang H, Zhao X, Li J, Cai H, Deng XW, Li L (2014). MicroRNA408 is critical for the HY5-SPL7 gene network that mediates the coordinated response to light and copper. Plant Cell 26:4933-4953.

9)      Wang L#, Li J#, Zhou Q, Yang G, Ding XL, Li X, Cai CX, Zhang Z, Wei HY, Lu TH, Deng XW, Huang XH (2014). Rare earth elements activate endocytosis in plant cells. Proc Natl Acad Sci USA 111: 12936-12941. (# equal contribution)

10)  Xu D, Lin F, Jiang Y, Huang X, Li J, Ling J, Hettiarachchi C, Tellgren-Roth C, Holm M, Deng XW (2014). The RING-finger E3 ubiquitin ligase COP1 SUPPRESSOR1 negatively regulates COP1 abundance in maintaining COP1 homeostasis in dark-grown Arabidopsis seedlings. Plant Cell 26:1981-1991.

11)  Xu D#, Li J#, Gangappaa SN, Hettiarachchia C, Lin F, Andersson MX, Jiang Y, Deng XW, Holm M (2014). Convergence of light and ABA signaling on the ABI5 promoter. PLoS Genet 10:e1004197. (# equal contribution)

12)  Zhu D, Wu Z, Cao G, Li J, Wei J, Tsuge T, Gu H, Aoyama T and Qu LJ (2014). TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes. Mol Plant 7:601-615.

13)  Li J, Yang L, Jin D, Nezames CD, Terzaghi W and Deng XW (2013). UV-B-induced photomorphogenesis in Arabidopsis. Protein Cell 7: 485–492.

14)  He G#, Chen B#, Wang X#, Li X#, Li J#, He H, Yang M, Lu L, Qi Y, Wang X and Deng XW (2013). Conservation and divergence of transcriptomic and epigenomic variation in maize hybrids. Genome Biol 14:R57. (# equal contribution)

15)  Li B, Duan H, Li J, Deng XW, Yin W and Xia X (2013). Global identification of miRNAs and targets in Populus euphratica under salt stress. Plant Mol Biol 81: 525-539.

16)  Huang X, Ouyang X, Yang P, Lau OS, Li G, Li J, Chen H and Deng XW (2012). Arabidopsis FHY3 and HY5 positively mediate induction of COP1 transcription in response to photomorphogenic UV-B light. Plant Cell 24:4590-4606.

17)  Zhong S, Shi H, Xue C, Wang L, Xi Y, Li J, Quail PH, Deng XW and Guo H (2012). A molecular framework of light-controlled phytohormone action in Arabidopsis. Curr Biol 22:1530-1535.

This paper was featured in:

Lorrain S and Fankhauser C. Plant development: should I stop or should I grow? Curr Biol 22: R645-647.

18)  Chen F, Shi X, Chen L, Dai M, Zhou Z, Shen Y, Li J, Li G, Wei N and Deng XW (2012). Phosphorylation of FAR-RED ELONGATED HYPOCOTYL1 is a key mechanism defining signaling dynamics of phytochrome A under red and far-red light. Plant Cell 24:1907-1920.

19)  Yang DL, Yao J, Mei CS, Tong XH, Zeng LJ, Li Q, Xiao LT, Sun TP, Li J, Deng XW, Lee CM, Thomashow MF, Yang Y, He Z and He SY (2012). Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade. Proc Natl Acad Sci USA 109:E1192-1200.

20)  Li J, Terzaghi W and Deng XW (2012). Genomic basis for light control of plant development. Protein Cell 3:106-116.

21)  Shen H#, He H#, Li J#, Chen W, Wang X, Guo L, Peng Z, He G, Zhong S, Qi Y, Terzaghi W and Deng XW (2012). Genome-wide analysis of DNA methylation and gene expression changes in two Arabidopsis ecotypes and their reciprocal hybrids. Plant Cell 24:875-892. (# equal contribution)

This paper was featured in:

Hofmann NR. A global view of hybrid vigor: DNA methylation, small RNAs, and gene expression. Plant Cell 24:841.

“Author Profile” could be found in:

http://www.plantcell.org/content/24/3/875/suppl/DC2

22)  Li J, Li G, Wang H and Deng XW (2011). Phytochrome signaling mechanisms. Arabidopsis Book 9:e148. doi: 10.1199/tab.0148.

23)  Ouyang X#, Li J#, Li G#, Li B#, Chen B, Shen H, Huang X, Mo X, Wan X, Lin R, Li S, Wang H and Deng XW (2011). Genome-wide binding site analysis of FAR-RED ELONGATED HYPOCOTYL3 reveals its novel function in Arabidopsis development. Plant Cell 23:2514-2535. (# equal contribution)

24)  Li G, Siddiqui H, Teng Y, Lin R, Wan X, Li J, Lau OS, Ouyang X, Dai M, Wan J, Devlin PF, Deng XW and Wang H (2011). Coordinated transcriptional regulation underlying the circadian clock in Arabidopsis. Nat Cell Biol 13:616-622.

25)  Li J, Li G, Gao S, Martinez C, He G, Zhou Z, Huang X, Lee JH, Zhang H, Shen Y, Wang H and Deng XW (2010). Arabidopsis transcription factor ELONGATED HYPOCOTYL5 plays a role in the feedback regulation of phytochrome A signaling. Plant Cell 22:3634-3649.

26)  Lee JH, Yoon HJ, Terzaghi W, Martinez C, Dai M, Li J, Byun MO and Deng XW (2010). DWA1 and DWA2, two Arabidopsis DWD protein components of CUL4-based E3 ligases, act together as negative regulators in ABA signal transduction. Plant Cell 22:1716-1732.

27)  Chen H, Huang X, Gusmaroli G, Terzaghi W, Lau OS, Yanagawa Y, Zhang Y, Li J, Lee JH, Zhu D and Deng XW (2010). Arabidopsis CULLIN4-Damaged DNA Binding Protein 1 interacts with CONSTITUTIVELY PHOTOMORPHOGENIC1-SUPPRESSOR OF PHYA complexes to regulate photomorphogenesis and flowering time. Plant Cell 22:108-123.

28)  Shen Y, Zhou Z, Feng S, Li J, Tan-Wilson A, Qu LJ, Wang H and Deng XW (2009). Phytochrome A mediates rapid red light–induced phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a low fluence response. Plant Cell 21:494-506.

29)  Saijo Y, Zhu D, Li J, Rubio V, Zhou Z, Shen Y, Hoecker U, Wang H and Deng XW (2008). Arabidopsis COP1/SPA1 complex and FHY1/FHY3 associate with distinct phosphorylated forms of phytochrome A in balancing light signaling. Mol Cell 31:607-613.

30)  Yang X, Li J, Pei M, Gu H, Chen Z and Qu LJ (2007). Over-expression of a flower-specific transcription factor gene AtMYB24 causes aberrant anther development. Plant Cell Rep 26:219-228.

31)  Li J, Li X, Guo L, Lu F, Feng X, He K, Wei L, Chen Z, Qu LJ and Gu H (2006). A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice. J Exp Bot 57:1263-1273.

32)  Li J, Yang X, Wang Y, Li X, Gao Z, Pei M, Chen Z, Qu LJ and Gu H (2006). Two groups of MYB transcription factors share a motif which enhances trans-activation activity. Biochem Biophys Res Commun 341:1155-1163.

33)  Chen Y, Yang X, He K, Liu M, Li J, Gao Z, Lin Z, Zhang Y, Wang X, Qiu X, Shen Y, Zhang L, Deng X, Luo J, Deng XW, Chen Z, Gu H and Qu LJ (2006). The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol 60:107-124.

34)  Qin G, Kang D, Dong Y, Shen Y, Zhang L, Deng X, Zhang Y, Li S, Chen N, Niu W, Chen C, Liu P, Chen H, Li J, Ren Y, Gu H, Deng XW, Qu LJ and Chen Z (2003). Obtaining and analysis of flanking sequences from T-DNA transformants of Arabidopsis. Plant Sci 165:941-949.