课题组长

English

张舒群,博士,教授,国家“千人计划”入选者。
联系电话:86-571-88982417(O);86-571-88982466(L)
E-mail:clszhangsq@zju.edu.cn
研究方向:MAPK级联信号在植物抗病抗逆及生长发育中的调控机理研究

教育经历
1990.08-1993.12,University of Texas,Plant Sciences
1988.09-1990.07,University of Texas,Visiting Scholar
1985.09-1988.08,中科院水生生物研究所,博士生
1981.09-1985.06,南京大学,生物系,学士

工作经历
2011.06-Present,浙江大学,植物科学研究所,教授
2009.09-Present,University of Missouri,Department of Biochemistry,Professor
2003.09-2009.08,University of Missouri,Associate Professor
1998.09-2003.08,University of Missouri,Assistant Professor
1995.03-1998.08,Rutgers University,Post-doc,Daniel Klessig Lab
1994.01-1994.12,University of Texas,Post-doc,Mona Mehdy Lab

主要研究方向
本课题组长期致力于植物生物化学和分子生物学领域的研究,在发掘丝裂原活化蛋白激酶(Mitogen-Activated Protein Kinases, MAPKs)信号系统的成员,及其对植物生长、发育、抗病及其他逆境反应等方面的功能和分子机制等领域开展系统研究,共发表SCI论文60余篇,论文平均影响因子8.5,累计他引次数8600余次,H指数41。
1. MAPK级联信号对植物生长发育的调控
课题组多年系统研究发现MPK3/MPK6级联信号调控了多个重要植物生长发育过程,包括合子的第一次不对称分裂、胚胎细胞分化、胚珠的珠被发育、气孔分化、叶柄伸长、花器官脱落、花粉管导向、花粉发育、花序形态建成等。今后的研究将致力于鉴定MAPK调控生长发育各个过程的上游信号组分(比如类受体蛋白激酶)、下游底物(比如转录因子)及具体分子机制研究。
2. MAPK级联信号对植物抗病抗逆调控的机理研究
MAPK级联信号通过调控不同的底物参与了植物的抗病过程中的不同反应的调节。我们在国际上鉴定了第一个植物MAPK底物蛋白,乙烯合成限速酶ACS2和ACS6。MPK3/MPK6介导的磷酸化能够提高ACS2和ACS6蛋白的稳定性并促使其在细胞中积累,从而诱导了乙烯的合成。此外,我们发现MKK4/5-MPK3/6级联通过磷酸化WRKY33转录因子调控了ACS2/ACS6及植保素Camalexin合成基因的表达。最近我们又鉴定出一个新的MAPK底物ERF6,ERF6的磷酸化在植物抗病中起重要作用。我们仍将致力于MAPK其他底物的研究及MAPK所调控的信号通路在植物抗病及其他逆境信号中的功能研究。
发表论文/论著    (*通讯作者)
发表文章 (*通讯作者):
Xu, J. and Zhang, S.* (2015) Mitogen-activated protein kinase cascades in signaling plant growth and development. Trends in Plant Science 20: 56-64.
Xu, J. and Zhang, S.* (2015) Ethylene biosynthesis and regulation in plants. Ethylene in Plants, Springer (Book chapter).
Guan, Y., Meng X., Khanna R., LaMontagne, E., Liu, Y., Zhang S.* (2014) Phosphorylation of a WRKY transcription factor by MAPKs is required for pollen development and function in Arabidopsis. PLoS Genetics 5, e1004384.
Guan, Y., Lu, J., Xu, J., McClure B., Zhang S.* (2014) Two mitogen-activated protein kinases, MPK3 and MPK6, are required for funicular guidance of pollen tubes in Arabidopsis. Plant Physiology 165, 528-533.
Xu, J.*, Xie, J., Yan, C., Zou, X., Ren, D., Zhang S.* (2014) A chemical genetic approach demonstrates that MPK3/MPK6 activation and NADPH oxidase-mediated oxidative burst are two independent signaling events in plant immunity. Plant Journal 77, 222-234.
Xu, J.*, Yang, K., Yoo, S., Liu, Y., Ren, D., Zhang, S. (2014) Reactive oxygen species in signaling the transcriptional activation of WIPK expression in tobacco. Plant Cell & Environment 37, 1614-1625.
Xu, J.* and Zhang, S.* (2014) Regulation of ethylene biosynthesis and signaling by protein kinases and phosphatase. Molecular Plant 7, 939-942. (spotlight paper)
Xu, J.* and Zhang, S.* (2014) RNA interference of plant MAPK cascades for functional studies. Plant MAP Kinases, Methods in Molecular Biology, Springer. 1171, 91-103 (Book chapter)
Meng, X., Xu, J., He, YX., Yang, K., Mordorski, B., Liu, Y., Zhang S.* (2013) Phosphorylation of an ERF transcription factor by Arabidopsis MPK3/MPK6 regulates plant defense gene induction and fungal resistance. Plant Cell 3, 1126-1142.
Meng, X. and Zhang, S.* (2013). MAPK cascades in plant disease resistance signaling. Annual Review of Phytopathology 51, 245-266.
Meng, X., Wang, H., He, Y., Liu, Y., Walker, J.C., Torii, K.U., and Zhang, S.* (2012). A MAPK cascade downstream of ERECTA receptor-like protein kinase regulates Arabidopsis inflorescence architecture by promoting localized cell proliferation. Plant Cell 24: 4948-4960.
Li, G., Meng, X., Wang, R., Mao, G., Han, L., Liu, Y., and Zhang, S.* (2012). Dual-level regulation of ACC synthase activity by MPK3/MPK6 cascade and its downstream WRKY transcription factor during ethylene induction in Arabidopsis. PLoS Genetics 8: e1002767.
Mao, G., Meng, X., Liu, Y., Zheng, Z., Chen, Z., and Zhang, S.* (2011) Phosphorylation of a WRKY transcription factor by two pathogen-responsive MAPKs drives phytoalexin biosynthesis in Arabidopsis. Plant Cell 23: 1639-1653.
Mao, G., Wang, R., Guan, Y.F., Liu, Y., and Zhang, S.* (2011) Sulfurtransferase 1 plays essential roles in embryo and seed development in Arabidopsis thaliana. Journal of Biological Chemistry 286, 7548-7557.
Han, L., Li, G.-J., Yang, K.-Y., Mao. G., Wang, R., Liu, Y., and Zhang, S.* (2010) Arabidopsis mitogen-activated protein kinase cascade in regulating Botrytis cinerea-induced ethylene production. Plant Journal 64: 114–127.
An, F., Zhao, Q., Ji, Y., Li, W., Jiang, Z., Yu, X., Zhang, C., Han, Y., He, W., Liu, Y., Zhang, S., Ecker, J.R., and Guo, H. (2010) Ethylene-induced stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 is mediated by proteasomal degradation of EIN3 binding F-box 1 and 2 that requires EIN2 in Arabidopsis. Plant Cell 22: 2384–2401.
Jang, E.-K., Min, K.-H., Kim, S.-H., Nam, S.-H., Zhang, S., Kim, Y.C., Cho, K.H., and Yang, K.-Y. (2009) Mitogen-activated protein kinase cascade in the signaling for polyamine biosynthesis in tobacco. Plant Cell Physiology 50: 658–664.
Beckers, G.J.M., Jaskiewicz, M., Liu, Y., Underwood, W.R., He, S.Y., Zhang, S., and Conrath, U. (2009) MAP kinases 3 and 6 are required for priming of stress responses in Arabidopsis. Plant Cell 21: 944–953.
Cho, S.K., Larue, C., Chevalier, D., Wang, H., Jinn, T.-L., Zhang, S.*, and Walker, J.C.* (2008) Regulation of floral organ abscission in Arabidopsis thaliana. Proceedings of the National Academy Sciences, USA 105:15629–15634. (Highlighted in PNAS "in this issue")
Ren, D., Liu, Y., Yang, K.-Y., Han, L., Mao, G., Glazebrook, J., and Zhang, S.* (2008) Fungal-responsive mitogen-activated protein kinase cascade in signaling phytoalexin biosynthesis in Arabidopsis. Proceedings of the National Academy Sciences, USA. 105: 5638–5643.
Wang, W., Liu, Y., Bruffett, K., Lee, J., Hause, G., Walker, J.C., and Zhang, S.* (2008) Haplo-insufficiency of MPK3 in MPK6 mutant background uncovers a novel function of these two MAPKs in Arabidopsis ovule development. Plant Cell 20: 602–613.
Hord, C.L.H., Sun, Y., Pillitteri, L.J., Torii, K.U., Wang, H., Zhang, S., and Ma H. (2008) The mitogen-activated protein kinases, MPK3 and MPK6, and the ERECTA and related receptor-like kinases are important for Arabidopsis anther development. Molecular Plant 1:645–658.
Zhang, S.* (2008) Mitogen-activated protein kinase cascades in plant intracellular signaling. In Annual Plant Reviews, Volume 33: Intracellular Signaling in Plants, edited by Z. Yang. Oxford: Wiley-Blackwell. (ISBN: 9781405160025). 
Joo, S., Liu, Y., Lueth, A., and Zhang, S.* (2008) MAPK phosphorylation-induced stabilization of ACS6 protein is mediated by non-catalytic C-terminal domain, which also contains the cis-determinant for rapid degradation by the 26S proteasome pathway. Plant Journal 54: 129–140.
Underwood, W., Zhang, S., and He, S.-Y. (2007) The Pseudomonas syringae type III effector tyrosine phosphatase HopAO1 suppresses innate immunity in Arabidopsis thaliana. Plant Journal 52: 658–672
Liu, Y., Ren, D., Pike, S., Pallardy, S., Gassmann, W., and Zhang, S.* (2007) Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade. Plant Journal 51: 941–954. 
Wang, H., Ngwenyama, N., Liu, Y., Walker, J.C., and Zhang, S.* (2007) Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis. Plant Cell 19: 63–73.
Mino, M., Kubota, M., Nogi, T., Zhang, S., and Inoue, M. (2007) Hybrid lethality in interspecific Nicotiana gossei x N. tabacum involves a MAP-kinases signalling cascade. Plant Biology 9: 366–373.
Suarez-Rodriguez, M.C., Adams-Phillips, L., Liu, Y., Wang, H., Su, S.-H., Jester, P.J., Zhang, S., Bent, A.F., and Krysan, P.J. (2007) MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants. Plant Physiology 143: 661–669.
Ren, D., Yang, K.-Y., Li, G., Liu, Y., and Zhang, S.* (2006) Activation of Ntf4, a tobacco mitogen-activated protein kinase, during plant defense response and its involvement in hypersensitive response-like cell death. Plant Physiology 141: 1482–1493. 
Hamel, L.-P., Nicole, M.-C., Sritubtim, S., Morency, M.-J., Ellis, M., Ehlting, J., Beaudoin, N., Barbazuk, B., Klessig, D., Lee, J., Martin, G., Mundy, J., Ohashi, Y., Scheel, D., Sheen, J., Xing, T., Zhang, S., Seguin, A., and Ellis, B.E. (2006) Ancient signals: comparative genomics of plant MAPK and MAPKK gene families. Trends in Plant Science 11: 192–198.
Liu, Y. and Zhang, S.* (2004). Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis. Plant Cell 16: 3386–3399. (Featured in The Plant Cell's “In this issue” and on the Science STKE website [First plant MAP kinase substrate. http://stke.sciencemag.org/cgi/content/abstract/sigtrans;2004/263/tw447], and also cited as an ISI Fast Breaking Paper, Citing URL: http://www.esi-topics.com/fbp/2005/december05-ShuqunZhang.html).
Kim, C.Y., and Zhang, S.* (2004) Activation of a mitogen-activated protein kinase cascade induces WRKY family of transcription factors and defense genes in tobacco. Plant Journal 38: 142–151. (Corresponding author)
Wan, J., Zhang, S. and Stacey, G. (2004). Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin. Molecular Plant Pathology 5: 125–135.
Kim, C.Y., Liu, Y., Thorne, E.T., Yang, H., Fukushige, H., Gassmann, W., Hildebrand, D., Sharp, R.E., and Zhang, S.* (2003) Activation of a stress-responsive mitogen-activated protein kinase cascade induces the biosynthesis of ethylene in plants. Plant Cell 15: 2707–2718.
Liu, Y., Jin, H., Yang, K.-Y., Kim, C.-Y., Baker, B., and Zhang, S.* (2003) Interaction between two mitogen-activated protein kinases during tobacco defense signaling. Plant Journal 34: 149–160.
Jin, H., Liu, Y., Yang, K.-Y., Kim, C.-Y., Baker, B., and Zhang, S.* (2003) Function of a mitogen-activated protein kinase pathway in N gene-mediated resistance in tobacco. Plant Journal 33: 719–731. (Noted as an ISI Fast Moving Front article, Citing URL: http://www.esi-topics.com/fmf/2005/september05-ShuqunZhang.html).
Yang, K.-Y., Blee, K.A., Zhang, S., and Anderson, A.J. (2002) OxycomTM treatment suppresses Pseudomonas syringae infection and activates a mitogen-activated protein kinase pathway in tobacco. Physiological and Molecular Plant Pathology 61: 249–256.
Jin, H., Axtell, M.J., Dahlbeck, D., Ekwenna, O., Zhang, S., Staskawicz, B., and Baker, B. (2002). NPK1, an MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants. Developmental Cell 3: 291–297.
MAPK Group (2002) Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends in Plant Science 7: 301–308.
Ren, D., Yang, H., and Zhang, S.* (2002) Cell death mediated by MAPK is associated with hydrogen peroxide production in Arabidopsis. Journal Biological Chemistry 277: 559–565.
Yang, K.-Y., Liu, Y., and Zhang, S.* (2001). Activation of a mitogen activated protein kinase pathway is involved in disease resistance in tobacco. Proceedings of the National Academy Sciences, USA. 98,741–746.
Zhang, S.*, and Klessig, D.F. (2001). MAPK cascades in plant defense signaling. Trends in Plant Science 6, 520–527.
Zhang, S., and Klessig, D.F.* (1998). N resistance gene-mediated de novo synthesis and activation of a tobacco MAP kinase by TMV infection. Proceedings of the National Academy Sciences, USA. 95, 7433–7438.
Zhang, S., and Klessig, D.F.* (1998). The tobacco wounding-activated MAP kinase is encoded by SIPK. Proceedings of the National Academy Sciences, USA. 95, 7225–7230.
Zhang, S., Du, H., and Klessig, D.F.* (1998). Activation of tobacco SIP kinase by both a cell wall-derived carbohydrate elicitor and purified proteinaceous elicitins from Phytophthoraspp. Plant Cell 10, 435–449.
Zhang, S., and Klessig, D.F.* (1997). Salicylic acid activates a 48 kD MAP kinase in tobacco. Plant Cell 9,809–824.