课题组长

English

武维华,博士,教授

联系电话:86-10-62731103(O)86-10-62731322(L)
E-mail
 whwu@cau.edu.cn   
研究方向:植物钾、磷高效和抗逆(干旱、高盐)机理研究
主要研究方向

1 植物响应低钾胁迫的分子机制研究。
本实验室通过对化学诱变的拟南芥突变体进行大规模筛选,分别获得了具有明显低钾敏感表型和耐低钾表型的拟南芥突变体lks1lkt1LKS1编码丝氨酸/苏氨酸蛋白激酶CIPK23LKS1与上游钙感受器蛋白CBL1/9互作,通过磷酸化作用激活质膜上钾离子通道蛋白AKT1的活性,进而促进拟南芥根部的钾离子吸收(Xu et al., 2006,Cell)。进一步研究显示,在水稻和玉米中也可能存在类似的CBL-CIPK蛋白复合体,它们也可以正向调控钾离子通道蛋白的活性,从而影响水稻和玉米的钾离子吸收。另一方面,我们发现钾离子通道蛋白亚基AtKC1LKT1)和钙感受器CBL10都可以直接与AKT1互作,并通过调控AKT1通道的活性而影响拟南芥的钾离子吸收(Wang et al., 2010, Cell Res; Ren et al., 2013, Plant J)。说明,CBL1/9-CIPK23LKS1)、AtKC1LKT1)和CBL10可能形成分子调控网络,精细调控AKT1介导的钾吸收过程。

2
 植物响应低磷胁迫的分子机制研究。
在低磷胁迫条件下,植物许多基因的表达发生明显变化,说明转录因子在植物响应低磷胁迫过程中起重要作用。我们的研究结果表明拟南芥WRKY6WRKY42参与调控植物的磷转运。在正常供磷条件下,WRKY6直接结合到PHO1启动子上,抑制PHO1基因的表达,抑制磷从根部向地上部转运;在低磷胁迫条件下,WRKY6蛋白被降解,WRKY6PHO1的抑制作用被解除(Chen et al., 2009, Plant Cell)。WRKY42也能结合到PHO1启动子进而抑制PHO1基因的表达,同时WRKY6WRKY42能互相负调控基因的转录水平。除了WRKY6WRKY42,我们还发现其他转录因子参与植物响应低磷胁迫。

3
 钙依赖蛋白激酶参与离子通道调控的机制研究。
实验室研究发现钙依赖蛋白激酶CPK8CPK10参与拟南芥对干旱胁迫的响应。cpk8cpk10的单突变体均表现出干旱敏感的表型。膜片钳实验表明,ABACa2+能够抑制野生型拟南芥气孔保卫细胞原生质体的内向钾通道电流,但却不能抑制cpk8cpk10突变体的内向钾通道电流(Zou et al., 2010, Plant Physiol)。说明CPK8CPK10可能通过参与调控气孔内向钾通道而影响气孔的运动。另一方面,我们还发现钙依赖蛋白激酶CPK11CPK24参与了Ca2+对花粉管质膜内向钾电流的抑制作用。在拟南芥野生型花粉管原生质体中,胞质Ca2+浓度升高可以显著抑制质膜内向钾电流。然而,在cpk11cpk24突变体中,胞质Ca2+浓度升高对花粉管内向钾电流的抑制作用完全消失。说明,在拟南芥花粉管中,CPK11CPK24组成的级联信号系统介导了Ca2+信号对质膜内向钾通道SPIK的抑制作用,从而调控了花粉管生长(Zhao et al., 2013, Plant Cell)。

发表论文/论著    (*通讯作者)
Wang XP, Chen LM, Liu WX, Shen LK, Wang FL, Zhou Y, Zhang Z, Wu W-H, Wang Y* (2016) AtKC1 and CIPK23 synergistically modulate AKT1-mediated low potassium stress responses in Arabidopsis. Plant Physiol. 170:2264-2277. 
Han M, Wu W, Wu W-H, Wang Y* (2016) Potassium transporter KUP7 is involved in K+ acquisition and translocation in Arabidopsis root under K+-limited conditions. Mol Plant. 9:437-446.
Wang Y, Wu W-H*(2015) Genetic approaches for improvement of the crop potassium acquisition and utilization efficiency. Curr. Opin. Plant Biol. 25:46-52.
Zou J-J, Li X-D, Ratnasekera D, Wang C, Liu W-X, Song L-F, Zhang W-Z, Wu W-H*(2015) Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 function in abscisicacid-mediated signaling and H2O2homeostasis in stomatalguard cells under drought stress. Plant Cell. 27:1445-1460.
Su T, Xu Q, Zhang F-C, Chen Y, Li L-Q, Wu W-H, Chen Y-F* (2015) WRKY42 modulates phosphate homeostasis through regulating phosphate translocation and acquisition in Arabidopsis. Plant Phsyiol. 167:1579-1591.
Li J, Long Y, Qi G-N, Li J, Xu Z-J, Wu W-H and Wang Y* (2014) The Os-AKT1 channel is critical for K+ uptake in rice roots and is modulated by the rice CBL1-CIPK23 complex. Plant Cell. 26:3387-3402.
Feng C-Z, Chen Y, Wang C, Kong Y-H, Wu W-H, Chen Y-F* (2014) Arabidopsis RAV1 transcription factor, phosphorylated by SnRK2 kinases, regulates the expression of ABI3, ABI4, and ABI5 during seed germination and early seedling development. Plant J. 80:654-668.
Wang H, Xu Q, Kong Y-H, Chen Y, Duan J-Y, Wu W-H, Chen Y-F* (2014) Arabidopsis WRKY45 transcription factor activates PHOSPHATE TRANSPORTER1;1 expression in response to phosphate starvation. Plant Physiol. 164:2020-2029.
Liu W-X, Zhang F-C, Zhang W-Z, Song L-F, Wu W-H, Chen Y-F* (2013) Arabidopsis Di19 functions as a transcription factor and modulates PR1, PR2, and PR5 expression in response to drought stress. Mol. Plant. 6:1487-1502.
Wang Y and Wu W-H* (2013) Potassium transport and signaling in higher plants. Annu. Rev. Plant Biol. 64:451-476.
Liu L-L, Ren H-M, Chen L-Q, Wang Y and Wu W-H* (2013) A protein kinase, CIPK9, interacts with calcium sensor CBL3 and regulates K+ homeostasis under low-K+ stress in Arabidopsis. Plant Physiol. 161:266-277.
Ren X-L, Qi G-N, Feng H-Q, Zhao S, Zhao S-S, Wang Y and Wu W-H* (2013) Calcineurin B-like protein CBL10 directly interacts with AKT1 and modulates K+ homeostasis in Arabidopsis. Plant J. 74:258-266.
Zhao L-N, Shen L-K, Zhang W-Z, Zhang W, Wang Y and Wu W-H* (2013) Ca2+-dependent protein kinase11 and 24 modulate the activity of the inward rectifying K+ channels in Arabidopsis pollen tubes. Plant Cell. 25:649-661.
Zou J-J, Wei F-J, Wang C, Wu J-J, Ratnasekera D, Liu W-X and Wu W-H* (2010) Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol. 154:1232-1243.
Wang Y, He L, Li H-D, Xu J and Wu W-H* (2010) Potassium channel a-subunit AtKC1 negatively regulates AKT1-mediated K+ uptake in Arabidopsis roots under low-K+ stress. Cell Res. 20:826-837.
Wang Y and Wu W-H* (2010) Plant sensing and signaling in response to K+-deficiency. Mol. Plant. 3:280-287.
Chen Y-F, Li L-Q, Xu Q, Kong Y-H, Wang H and Wu W-H* (2009) The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis. Plant Cell. 21:3554-3566.
Zou J-J, Song L-F, Zhang W-Z, Wang Yi, Ruan S-L and Wu W-H* (2009) Comparative proteomic analysis of Arabidopsis mature pollen and germinated pollen. J.Integr. Plant Biol. 51:438-455.
Wang Y, Zhang W-Z, Song L-F, Zou J-J, Su Z and Wu W-H* (2008)Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol. 148:1201-1211.
Chen Y-F, Wang Y and Wu W-H* (2008) Membrane transporters for nitrogen, phosphate and potassium uptake in plants. J.Integr. Plant Biol. 50:835-848.
Ma S-Y and Wu W-H* (2007) AtCPK23 functions in Arabidopsis responses to drought and salt stresses. Plant Mol. Biol. 65:511-518.
Zhang W, Fan L-M and Wu W-H* (2007) Osmo-sensitive and stretch-activated calcium-permeable channels in Viciafaba guard cells are regulated by actin dynamics. Plant Physiol. 143:1140-1151.
Wang X-Y, Chen Y-F, Zou J-J and Wu W-H* (2007) Involvement of a cytoplasmic glyceraldehyde-3-phosphate dehydrogenase GapC-2 in Arabidopsis responses to low-phosphorous stress. Chinese Sci Bull. 52:1764-1770.
Xu J, Li H-D, Chen L-Q, Wang Y, Liu L-L, He L and Wu W-H* (2006) A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis. Cell.125:1347-1360.
Jiang J, Fan L-W and Wu W-H* (2005) Evidences for involvement of endofenouscAMP in Arabidopsis defense responses to Verticillium toxins. Cell Res. 15:585-592.
Wang Y-F, Fan L-M and Wu W-H* (2004) Ca2+-permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments. Plant Physiol. 136:3892-3904.
Fan L-M, Wang Y-F and Wu W-H* (2003) Outward K+ channels in Brassica chinensis pollen protoplasts are regulated by external and internal pH.Protoplasma. 220:143-152.
Fan L-M, Wang Y-F, Wang H and Wu W-H* (2001) In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts. J. Exp. Bot. 52:1603-1614.
Zhao S-Q, Lin C, Yang X-H and Wu W-H* (2001) Isolation and genetic analysis of Arabidopsis mutants with low-K+ tolerance. Acta Bot. Sinica. 43:105-107.
Fan L-M and Wu W-H* (2000) External pH regulates the inward K+ channels in Brassica pollen protoplasts. Prog. Nat. Sci. 10:68-73.
Jin X-C and Wu W-H* (1999) Involvement of cyclic AMP in ABA- and Ca2+-mediated signal transduction of stomatal regulation in Viciafaba. Plant Cell Physiol. 40:1127-1133.
Fan L-M and Wu W-H* (1999) Identification and characterization of the inward K+ channel in the plasma membrane of Brassica pollen protoplasts. Plant Cell Physiol. 40:859-865.
Liu P-R and Wu W-H* (1999) Physiological mechanisms of growth-inhibition by concentrated potassium in Dunaliellasalina. Acta Bot. Sinica. 41:617-623.
Yu C-J and Wu W-H* (1999) Identification and characterization of inward K+-channels in plamsma membrane of Arabidopsis root cortex cells. Sci. in China. 42:307-315.
Zhou X-M and Wu W-H* (1999) Regulation of the inward K+ channels in stomatal guard cells by cytoskeletal microtubules. Chinese Sci Bull. 44:919-922.
Wang X-Q, Wu W-H* and Assmann S (1998) Differential responses of abaxial and adaxial guard cells of broad bean to abscisic acid and calcium. Plant Physiol. 118:1421-1429.
Wang X-Q, Wu W-H* and Zhang J-S (1998) Evidences for regulation of the inward K+ channels by CDPK in Viciafaba guard cells. Acta Bot. Sinica. 40:1001-1009.
Wu W-H and Assmann S* (1995) Is ATP required for K+ channel activation in Vicia guard cells? Plant Physiol. 107:101-109.
Wu W-H* (1995) A novel cation channel in Viciafaba guard cell plasma membrane. ActaPhytophysiologicaSinica. 21:347-354.
Assmann S* and Wu W-H (1994) Inhibition of guard cell inward K+ channels by abscisic acid: Links and gaps in the signal transduction chain. In: Blatt MR, Leigh RA, & Sanders D eds., Membrane Transport in Plant and Fungi: Molecular Mechanisms and Control. The Company of Biologists Limited, Cambridge, England, pp193-202.
Schwartz A, Wu W-H, Tucker E and Assmann S* (1994) Inhibition of ineard K+ channels and stomatal response by abscisic acid: An intracellular locus of phytohormone action. Proc. Natl. Acad. Sci. USA. 91:4019-4023.
Wu W-H and Assmann S* (1994) A membrane-delimited pathway of G-protein regulation of the guard-cell inward K+ channel. Proc. Natl. Acad. Sci. USA. 91:6310-6314.
Wu W-H and Assmann S* (1993) Photosynthesis by guard cell chloroplasts of Viciafaba L.: Effects of factors associated with stomatal movement. Plant Physiol. 34:1015-1022.
Berkowitz G* and Wu W-H (1993) Magnesium, potassium flux and photosynthesis.Magnesium Res. 6:257-265.
Wu W-H and Berkowitz G* (1992) K+ stimulation of ATPase activity associated with the chloroplast inner envelope. Plant Physiol. 99:553-560.
Wu W-H and Berkowitz G* (1992) Stromal pH and photosynthesis are affected by electroneutral K+ and H+ exchange through chloroplast envelope ion channels. Plant Physiol. 98:666-672.
Wu W-H and Berkowitz G* (1991) Lidocaine and ATPase inhibitor interaction with the chloroplast envelope. Plant Physiol. 97:1551-1557.
Wu W-H, Peters J and Berkowitz G* (1991) Surface charge-mediated effects of Mg2+ on K+ flux across the chloroplast envelope are associated with regulation of stromal pH and photosynthesis. Plant Physiol. 97:580-587.