Yang, Shuhua    Yang Lab
Ph.D., Professor
86-10-62734838 (O); 86-10-62734390 (L)
Research Area: Molecular Mechanism of Plant Responses to Low Temperature

1998-2002       Ph.D., National University of Singapore, Singapore
1991-1994       M.S., Nankai University, Tianjin, China
1987-1991       B.S., Nankai University, Tianjin, China

2005-present    Professor, State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China
2002-2004       Postdoctoral Associate, Cornell University, Ithaca, NY, USA
1994-1998       Research Assistant, Nankai University, Tianjin, China

Research of Special Interest

Research Interest
Our laboratory mainly focuses on the study of molecular mechanism of plant responses to low temperatures. As one of important environmental factors, low temperatures restrict plant growth, development and distribution. How plants sense and respond to low temperatures is poorly understood. To understand the molecular mechanism underlying plants’ response to low temperatures, we are undertaking genetic, biochemical and molecular approaches to identify novel components essential for low temperature signaling pathway and investigate the functions of these components in cold responses. We are also investigating the crosstalk of cold signaling and plant hormone signaling,  as well as biotic stress signaling. We aim at a deeper understanding of how plants perceive and respond to cold stress.


Publications      *Correspondence Author

 (*Corresponding author; #These authors contributed equally)

    1.       Ding Y#, Shi Y#, Yang S* (2019) Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants. New Phytol Doi: 10.1111/nph.15696.
    2.       Ding Y, Lv J, Shi Y, Gao J, Hua J, Song C, Gong Z, Yang S* (2019) EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis. EMBO J 38: e99819. (Selected for F1000 Prime)
    3.       Shi Y#, Ding Y#, Yang S* (2018) Molecular regulation of CBF signaling in cold acclimation. Trends Plant Sci 23: 623-637.
    4.       Ding Y, Jia Y, Shi Y, Song C, Gong Z, Yang S* (2018) OST1-mediated BTF3L phosphorylation positively regulates CBFs during plant cold responses. EMBO J 37: e98228.
    5.       Liu J#, Shi Y#, Yang S* (2018) Insights into the regulation of C-repeat binding factors in plant cold signaling. J Integr Plant Biol 60: 780-795.
    6.        Huang X, Hou L, Meng J, You H, Li Z, Gong Z, Yang S, Shi Y*(2018) The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis. Mol Plant 11: 970-982.
    7.        Wang K#, He J#, Zhao Y, Wu T, Zhou X, Ding Y, Kong L, Wang X, Wang Y, Li J, Song CP, Wang B, Yang S, Zhu JK, Gong Z* (2018) EAR1 negatively regulates ABA signaling by enhancing 2C protein phosphatase activity. Plant Cell 30: 815-834.
    8.       Wang X, Guo C, Peng J, Li C, Wan F, Zhang S, Zhou Y, Yan Y, Qi L, Sun K, Yang S, Gong Z, Li J* (2018) ABRE-BINDING FACTORS play a role in the feedback regulation of ABA signaling by mediating rapid ABA induction of ABA co-receptor genes. New Phytol 221: 341-355.
    9.  Wei D, Liu M, Chen H, Zheng Y, Liu Y, Wang X, Yang S, Zhou M*, Lin J* (2018) INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis. PLoS Genet 14: e1007695.
    10.     Feng Y, Liu J, Zhai L, Gan Z, Zhang G, Yang S, Wang Y, Wu T, Zhang X, Xu X, Han Z* (2018)  Natural variation in cytokinin maintenance improves salt tolerance in apple rootstocks. Plant Cell Environ doi: 10.1111/pce.13403.
    11.     Li H, Ding Y, Shi Y, Zhang X, Zhang S, Gong Z, Yang S* (2017) MPK3- and MPK6-mediated ICE1 phosphorylation negatively regulates ICE1 stability and freezing tolerance in Arabidopsis. Dev Cell 43: 630-642. (Highlighted with a Preview article in Dev Cell and a Spotlight article in Trend Plant Sci; Selected for F1000 Prime)
    12.     Jiang B#, Shi Y#, Zhang X, Xin X, Qi L, Guo H, Li J*, Yang S* (2017) PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis. Proc Natl Acad Sci USA 114: E6695-E6702. (Highlighted with a News & Views in Nat Plant and a Spotlight article in Trend Plant Sci)
    13.     Liu Z, Jia Y, Ding Y, Shi Y, Zhang X, Li Z, Guo Y, Gong Z, Yang S* (2017) Plasma membrane CRPK1-mediated phosphorylation of 14-3-3 proteins induces their nuclear import to fine-tune CBF signaling during cold response. Mol Cell 66: 117-128. (Highlighted with a Preview article in Mol Cell; Selected for F1000 Prime)
    14.     Li H#, Ye K#, Shi Y, Zhang X, Yang S* (2017) BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis. Mol Plant 10: 545-559. (Highlighted with a Spotlight article in Mol Plant)
    15.     Zhang Z#, Li J#, Pan Y#, Li J#, Zhou L#, Shi H#, Zeng Y#, Guo H, Yang S, Zheng W, Yu J, Sun X, Li G, Ding Y, Ma L, Shen S, Dai L, Zhang H, Yang S, Guo Y, Li Z* (2017) Natural variation in CTB4a enhances rice adaptation to cold habitats. Nat Commun 8:14788. doi: 10.1038/ncomms14788.
    16.     Zhang Y#, Wang Y#, Liu J, Ding Y, Wang S, Zhang X, Liu Y, Yang S* (2017) Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3. New Phytol 213:1330-1345.
    17.     Tong M, Kotur T, Liang W, Vogelmann K, Kleine T, Leister D, Brieske C, Yang S, Lüdke D, Wiermer M, Zhang Y, Li X*, Hoth S*. (2017) E3 ligase SAUL1 serves as a positive regulator of PAMP-triggered immunity and its homeostasis is monitored by immune receptor SOC3.  New Phytol 215:1516-1532.
    18.     Huang X, Zhang X, Gong Z, Yang S, Shi Y* (2017) ABI4 represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis. Plant J 89: 354-365.
    19.     Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S* (2016) The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol 212: 345-353. (Highlighted with a Commentary article in New Phytol)
    20.     Yu F, Lou L, Tian M, Li Q, Ding Y, Wu Y, Belda-Palazon B, Rodrigue P L, Yang S, Xie Q* (2016) ESCRT-I component VPS23A affects ABA signaling by recognizing ABA receptors for endosomal degradation. Mol Plant 9: 1570-1582.
    21.     Ding Y, Li H, Zhang X, Xie Q, Gong Z, Yang S* (2015) OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis. Dev Cell 32: 278-289. (Highlighted with a Preview article in Dev Cell; Highlighted with an article in SCIENCE CHINA Life Sciences; 植物学报热点评)
    22.     Liu J#, Yang H#, Bao F, Ao K, Zhang X, Zhang Y, Yang S* (2015) IBR5 modulates temperature-dependent, R protein CHS3-mediated defense responses in Arabidopsis. PLoS Genet 11, e1005584.
    23.     Kong L, Cheng J, Zhu Y, Ding Y, Meng J, Chen Z, Xie Q, Guo Y, Li J, Yang S, Gong Z* (2015) Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases. Nat Commun 6: 8630. doi: 10.1038/ncomms9630.
    24.     Ji H, Wang Y, Cloix C, Li K, Jenkin G, Wang S, Shang Z, Shi Y, Yang S, Li X* (2015) The Arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis. PLoS Genet 11, e1005471.
    25.     Shi Y#, Ding Y#, Yang S* (2015) Cold signal transduction and its interplay with phytohormones during cold acclimation. Plant Cell Physiol 56: 7-15.
    26.     Bao F, Huang X, Zhu C, Zhang X, Li X, Yang S* (2014) Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol 202: 1320-1334.
    27.     Song S, Huang H, Gao H, Wang J, Wu D, Liu X, Yang S, Zhai Q, Li C, Qi T, Xie D* (2014) Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell 26: 263-279.
    28.     Wang Y, Zhang Y, Wang Z, Zhang X, Yang S* (2013) A missense mutation in CHS1, a TIR-NB protein, induces chilling sensitivity in Arabidopsis. Plant J 75: 553-565.
    29.     Shi Y, Wang Z, Meng P, Tian S, Zhang X, Yang S* (2013) The glutamate carboxypeptidase AMP1 mediates ABA and abiotic stress responses in Arabidopsis. New Phytol 199: 135-150.
    30.     Guo L, Yang H, Zhang X, Yang S* (2013) Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis. J Exp Bot 64: 1755-1767.
    31.     Li J#, Mu J#, Bai J#, Fu F, Zou T, An F, Zhang J, Jing H, Wang Q, Yang S, Zuo J* (2013) PARAQUAT RESISTANT 1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat. Plant Physiol 162: 470-483.
    32.     Shi Y#, Tian S#, Hou L, Huang X, Zhang X, Guo H, Yang S* (2012) Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. Plant Cell 24: 2578-2595.
    33.     Wang Z#, Meng P#, Zhang X, Ren D, Yang S* (2011) BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis. Plant J 67: 1081-1093.
    34.     Huang X, Li J, Bao F, Zhang X, Yang S* (2010) A gain-of-function mutation in the Arabidopsis disease resistance gene RPP4 confers sensitivity to low temperature. Plant Physiol 154: 796-809.
    35.     Yang H, Shi Y, Liu J, Guo L, Zhang X, Yang S* (2010) A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis. Plant J 63: 283-296.
    36.     Huang X, Li Y, Zhang X, Zuo J, Yang S* (2010) The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death. New Phytol 187: 301-312 (Cover story).
    37.     Liu D, Gong Q, Ma Y, Li P, Li J, Yang S, Yuan L, Yu Y, Pan D, Xu F, Wang NN* (2010) cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis. J Exp Bot 61: 1655-1669.
    38.     Huang X, Zhang X, Yang S* (2009) A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis. Cell Res 19: 1205-1216.
    39.     Gou M, Su N, Zheng J, Huai J, Wu G, Zhao J, He J, Tang D, Yang S, Wang G* (2009) An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis. Plant J 60: 757-770.
    40.     Li Y#, Yang S#, Yang H, Hua J* (2007) The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors. Mol Plant Microbe Interact 20: 1449-1456.
    41.     Yang H, Yang S, Li Y, Hua J* (2007) The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death. Plant Physiol 145: 135-146.
    42.     Chai MF, Wei PC, Chen QJ, An R, Chen YM, Chen J, Yang S*, Wang XC* (2006) NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis. Plant J 47: 665-674.
    43.     Yang S, Yang H, Grisafi P, Sanchatjate S, Fink GR, Sun Q, Hua J* (2006) The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis. Plant J 45: 166-179.
    44.     Yang S, and Hua J* (2004) A haplotype-specific resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis. Plant Cell 16: 1060-1071.
    45.     Yang S*, Yu H, Xu Y, Goh CJ* (2003) Investigation of cytokinin-deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1. FEBS Lett 555: 291-296.
    46.     Yang S, Yu H, Goh CJ* (2003) Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid. Plant Mol Biol 51: 237-248.
    47.     Yang S, Yu H, Goh CJ* (2002) Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter. J Exp Bot 53: 1899-1907.
    48.     Yu H, Yang S, Goh CJ* (2002) Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory elements. Plant Mol Biol 49: 225-237.
    49.     Yu H, Yang S, Goh CJ* (2001) Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1. Plant Cell Rep 20: 301-305.
    50.     Yu H, Yang S, Goh CJ* (2000) DOH1, a class 1 knox Gene, is required for maintenance of the basic plant architecture and floral transition in orchid. Plant Cell 12: 2143-2160.