FACULTY

Chinese
Xiao, Xing-Guo
Ph.D., Professor
Tel:86-10-62731324
E-mail:
xiaoxg@cau.edu.cn
Areas of Expertise:Plant engineering male sterility and Betalains biosynthesis
1995.5 – Present, Associate Professor and then Professor, China Agricultural University, Beijing, PRC
1994.1 –1995.3, Postdoc, CNRS-Paris VI, Paris, France
1982.1-1989.6, Zhengzhou Fruit Research Institute, CAAS, Zhengzhou, PRC,
1991.1 – 1994.1, Ph.D. Candidate, University of Western Brittany, Brest, France
1989.6-1990.7, Master Candidate, University of P. & M. Curie (Paris VI), Paris, France
1978.3-1981.12, Undergraduate student, Huazhong Agricultural University, Wuhan, PRC
Research of Special Interest

Plant engineering male sterility
Stable, complete and easy-recovery male sterility (MS) is imperative to produce F1 seeds. We are looking for methods to create such MS via gene engineering, and focus at “Intelligent MS” whose male fertility will be completely controlled by users, but not by environmental conditions such as temperature and/or light.

Betalain biosynthesis

Betalains are a class of water-soluble nitrogen-containing pigments, comprising red-violet betacyanins and yellow betaxanthins. They replace anthocyanins and serve the analogous functions in 13 families of the order Caryophyllales. Betalains and anthocyanins are mutually exclusive in the angiosperms, and they have never been observed in same plant. Betalain biosynthetic pathway has been widely speculated as that of melanin in animal, and the biosynthesis starts from oxidation of tyrosine catalyzed by tyrosinase although recently a new cytochrome P450 was reported to do the oxidation, too (Nat-Genet, 2012, 44(7): 816-820). We isolated and purified from red Swiss chard one of tyrosinases that could oxidize both monophenols and diphenols in vitro. Now, we are working at functional analysis of tyrosinase gene(s) cloned and are much interested in cloning all genes proposed involving in the betalain biosynthesis with the aim of reconstitution of betalain biosynthesis in vitro.



SDS-PAGE separation and verification of purified red Swiss chard tyrosinase(Food Chemistry, 2009, 117: 342-348)

Publications      *Correspondence Author

Xiao XG*, Han L, 2013, Plant guard cell-specific promoters and their use thereof. ZL201110336936.8
Xiao XG*, Gao ZJ, Han XH, Liu JB and Nie XL, 2012, Tyrosinase and its coding gene from betalain plant. ZL201010263562.7
Xiao XG*, Nie XL, Zhu JW and Geng AQ, 2010, Plant anther-specific promoters and their use thereof. ZL200710177905.6
Xiao XG*, Han XH, Zhu JW, Liu JB, Wang QW, 2010,  An induce-enhanced constitutive promoter and its use thereof. ZL200710177962.4
Xiao XG*, Zhao ZJ, Nie XL and Geng AQ, 2010, Plant flower-specific promoter and its use thereof. ZL200710118214.9
Xiao XG*, Geng AQ, Zhao ZJ and Nie XL, 2010,  A floral specific promoter its use thereof. ZL2007100989728.2
Xiao XG*, Liu JB, Yuan LJ, Gao ZJ and Han XH, 2009, A new method of indigo biosynthesis and use thereof. ZL200610114484.8
Xiao XG*, Gang AQ, Zhao ZJ and Nie XL, 2009,  A plant anther-specific promoter and its use thereof. ZL200610113024.3
Xiao XG*, Wang ZL, Cao KH, Han XH and Sun XH, 2007, Method for eliminating environment contamination by escaped transgene(s) and the expression vector. ZL200510108335.6
Xiao XG*, Sun XH, Cao KH, Wang ZL and Han XH, 2007, Method of artificially controlling the death of plant tissues and organs and the specific expression vector. ZL200510077616.X
Xiao XG*, Yan LF, Zhang AM, Li YH and Zhao GR, 2004, Methods for breeding plant male sterile line and its maintainer and the expression vector. ZL00109108.5

Han L, Han Y-N, Xiao XG* 2013, Truncated cotton subtilase promoter directs guard cell-specific expression of foreign genes in tobacco and Arabidopsis. PLoS ONE 8(3): e59802. doi:10.1371/journal.pone.0059802
Gao ZJ, Liu JB, Xiao XG* 2011, Purification and characterisation of polyphenol oxidase from leaves of Cleome gynandra L. Food Chem, 129: 1012-1018
Yuan LJ, Liu JB, Xiao XG* 2011, Biooxidation of indole and characteristics of the responsible enzymes. Afr J Biotechnol, 10(86): 19855-19863 (Dec. 30)
Nie XL, Zhu JW, Geng AG, Xiao XG*, 2010, Cloning and functional analysis in transgenic tobacco of a tapetum-specific promoter from Arabidopsis. Afr. J. Biotechnol. 2010, 9(41): 6826-6834 (IF: 0.565)
Gao ZJ, Han XH, Xiao XG*,2009, Purification and characterisation of polyphenol oxidase from red Swiss chard (Beta vulgaris subspecies cicla) leaves. Food Chem, 117: 342-348
Han XH, Gao ZJ, Xiao XG*, 2009, Enzymes and genes involved in the betalain biosynthesis in higher plants. Afr J Biotechnol, 8 (24): 6735-6744
Geng AQ, Zhao ZJ, Nie XL, Xiao XG*,2009, Expression analysis of four flower-specific promoters of Brassica spp. in the heterogeneous host tobacco. Afr J Biotechnol, 8 (20): 5193-5200
Han XH, Wang W, Xiao XG*, 2008, Microbial Biosynthesis and Biotransformation of Indigo and Indigo-like Pigments. Chin J Biotech, 2008, 24(6): 921-926.
Huang ZW, Feng YC, Chen D, Wu XJ,Huang SY, Wang XJ, Xiao XG, Li WH, Huang N, Gu LC, Zhong GM and Chai JJ, 2008, Structural Basis for Activation and Inhibition of the Secreted Chlamydia Protease CPAF. Cell Host & Microbe. 4: 529–542